Volume 1, Issue I, Page 31-38
Fabrication, Characterization and Antifungal Activity Studies of Silk Fibroin Hydrogel as a Potential Controlled Release of Fluconazole
Received: 02 April, 2021 || Accepted: 30 April, 2021 || Published: 3 May, 2021
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Article info Received: 02 April, 2021 Accepted: 30 April, 2021 Published: 3 May, 2021 Available in online: 03 May, 2021
*Corresponding author: Email: [email protected] |
Abstract Silk is a natural protein-based fiber derived from the Bombyx mori and certain insects. The research aim is to develop silk fibroin (SF) hydrogels loaded model drug fluconazole to obtain controlled release profile for better patient compliance. An eco-friendly technique was introduced to prepare fibroin hydrogel by treating three different organic solvent of ethanol, propanol, and glycerol by adding 2% (w/v) silk fibroin aqueous solution 37 ºC. The hydrogel having higher SF content showed more stability and slower degradation rate. Then, to prove the potential of SF hydrogel as carriers for drug delivery, fluconazole was absorbed in the hydrogel. All hydrogel released fluconazole in a controllable manner, possibly due to the hydrophobic interaction between fluconazole and crystalline domain of SF. The fibroin hydrogel was characterized by using Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Thermo gravimetrical Analysis (TGA), Differential scanning calorimetry (DSC). The encapsulation efficiency and release profile of fluconazole was studied by UV-VIS spectrometry. The surface morphology of the hydrogel was affected by the formulation conditions. Better encapsulation efficiency (85.5±1.67%, 73.4±1.76%, 71.6±1.35%, respectively) of fluconazole for F16, F17, F18 formulation were achieved when glycerol, propanol, ethanol was used in the formulation. The release profile showed an initial burst release for fluconazole then a controlled release for the next 20 hours. The antifungal activity of hydrogel incorporated drug showed a positive response against Aspergillus Niger pathogen. Therefore, silk fibroin hydrogels might be a good candidate for controlled topical delivery of fluconazole. Keywords: Silk fibroin, controlled release, hydrogels, drug delivery. |
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